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Samples were loaded at 20µg per lane onto a 15 SDS-polyacrylamide gel transferred to a PVDF membrane semi-dry and blocked with TBS containing TBST and 5 non-fat dry milk for 2 hrs at room temperature. Western blot analysis of HeLa whole cell lysate Lane 1 and GFP-transfected HeLa cell lysates Lane 2. 681259 NB Inc Thai Express 681626.
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